The focus of the lab is to unveil critical signaling pathways in cutaneous T cell lymphoma mycosis fungoides.
Mycosis fungoides (MF) is the most common cutaneous T cell lymphoma. Molecular mechanisms that control MF are still obscure. An optional mechanism involves the ubiquitin-proteasome system, that plays a central regulatory role in protein homeostasis and cell viability, thus may directly affect T cell survival.
The aim of the lab is to unveil ubiquitin enzymes that are crucial for T cell survival and to characterize their biological significance. We will first perform shRNA screen, to discover ubiquitin specific proteases (USPs) and ubiquitin ligases that are critical for MF viability. We will then select the most essential ligases, that their knockdown results in pronounced apoptosis. To identify the targets of the selected enzymes we will use liquid chromatography-tandem mass spectrometry (LC-MS/MS). Finally, we will validate our results in patient samples. We already have preliminary data suggesting the importance of several ubiquitin ligases for the viability of MF cells.